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上海研盟生物科技有限公司Anti-AU5 tag抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
英文名稱:Anti-AU5 tag antibody
中文名稱:AU5 tag標簽抗體
AU5 tag標簽抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
AU5 tag標簽抗體,Anti-AU5 tag抗體功能區(qū)的作用:
(1)VL和VH:結合抗原決定簇(FV區(qū))
(2)CL和CH:具有同種異型的遺傳標記
(3)CH2:結合補體
(4)CH3:結合Fc受體
抗原結合點由L鏈和H鏈超變區(qū)組成,與相應抗原上的表位互補,借助靜電力,氫鍵以及范德華力等次級鍵相結合。
★研盟生物★AU5 tag標簽抗體,Anti-AU5 tag抗體
免疫組化結果的判斷:
對免疫組化結果的判斷應持科學的慎重態(tài)度,要準確判斷陽性和陰性,排除假陽性和假陰性結果,必須嚴格對照實驗,對新發(fā)現(xiàn)的陽性結果,除有對照實驗結果之外,應進行多次重復實驗,可用幾種方法進行驗證。必須學會判斷特異性染色和非特異性染色,對初學者更為重要,否則會得出不科學的結論。特異性染色和非特異性染色的鑒別點主要在于特異性反應產(chǎn)物常分布于特定的部位。如胞漿內,也有分布在細胞核和細胞表面的,即具有結構性。特異性染色表現(xiàn)為在同一切片上呈現(xiàn)不同程度的陽性染色結果。非特異性染色表現(xiàn)為無一定的分布規(guī)律,常呈某一部位成片的均勻著色,細胞和周圍的結締組織均無區(qū)別的著色,或結締組織呈現(xiàn)很強的染色。非特異性染色常出現(xiàn)在干燥切片的邊緣,有刀痕或者折疊的部位。在過大的組織塊,中心固定不良也會導致非特異性染色。有時可見非特異性染色和特異性染色同時存在,由于過強的非特異性染色背景不但影響對特異性染色結果的觀察和記錄,而且令人對其特異性結果產(chǎn)生懷疑。
免疫組化的呈色深淺可反映抗原存在的數(shù)量,可作為定性、定位和定量的依據(jù)。
1.陽性細胞染色分布有三種類型:細胞漿、細胞核、細胞膜表面。大部分抗原見于細胞漿,可見于整個胞漿或部分胞漿
2.陽性細胞分布可分為灶型和彌漫性
3.由于細胞內含抗原量不同,所以染色強度不一。如果細胞之間染色強度相同,常提示其反應為非特異性
4.陽性細胞染色定位于單個細胞,且與陰性細胞相互交雜分布;而非特異性染色常不限于單個細胞,而是累及一片細胞。
5.切片邊緣、刀痕或褶皺區(qū)域,壞死或擠壓的細胞區(qū),膠原結締組織等,常表現(xiàn)為相同的陽性染色強度,不能用于判斷陽性。
簡單介紹:
產(chǎn)品別名:AU5 epitope tag; TDFYLK epitope tag; TDFYLK tag.
抗體來源:Rabbit or Mouse
保質期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. Modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. Also regulates many host cellular functions such as signaling pathways and apoptosis. Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Plays an important role in virus-mediated cell transformation leading to hepatocellular carcinomas. Interacts with, and activates STAT3 leading to cellular transformation. May repress the promoter of p53, and sequester CREB3 and SP110 isoform3/Sp110b in the cytoplasm. Also represses cell cycle negative regulating factor CDKN1A, thereby interrupting an important check point of normal cell cycle regulation. Targets transcription factors involved in the regulation of inflammatory responses and in the immune response: suppresses NK-kappaB activation, and activates AP-1. Mediates apoptotic pathways throught association with TNF-type receptors TNFRSF1A and LTBR, although its effect on death receptors-induced apoptosis remains controvertial. Enhances TRAIL mediated apoptosis, suggesting that it might play a role in mediated apoptosis, suggesting that it might play a role in immune-mediated liver cell injury. Secreted core protein is able to bind C1QR1 at the T-cell surface, resulting in down-regulation of T-lymphocytes proliferation. May transactivate human MYC, Rous sarcoma virus LTR, and SV40 promoters. May suppress the human FOS and HIV-1 LTR activity. May alter lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage.
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