上海研盟生物科技有限公司PEG3抗體-PEG3蛋白抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:PEG3蛋白抗體
英文名稱:Anti-PEG3
產品編號:BYK-1870R
產品別名:Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24; Kruppel-type zinc finger protein; paternally expressed 3; Paternally expressed gene 3; PEG3 protein; Pw1; Zn-finger protein Pw1; PEG3_HUMAN; Paternally-expressed gene 3 protein.
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PEG3抗體-PEG3蛋白抗體產品介紹:In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問題是二級結構的形成。雖然疏水肽鏈二級結構的形成更明顯,但除zui短的肽鏈外,此現象產生在幾乎所有肽鏈,與極性無關。所以,溶解多肽的*個原則是使用無菌蒸餾水或去離子水,當然無氧水。多肽溶液可能遇到細菌降解,為防止此情況的發生,應溶解在無菌的蒸餾水中或用0.45或0.2孔徑的濾膜過濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應溶于無氧的水中,無氧水可通過注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會引起溶液發熱或多肽降解;
3、若多肽含有多個堿性氨基酸,使用(1-10%)的乙酸水溶液:對于疏水性強的多肽,應使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發性的堿性緩沖液溶解。pH值在層析前必須調整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機溶劑的量必須很少否則將嚴重影響停留時間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或為中性肽時,DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過破壞多肽的二級結構而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會對其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達30%),滴加至多肽溶解;
d.反相層析法時,DMF將與洗脫液前鋒一起流出,根據入量的多少,峰值可能很高。大多數肽能在大量DMF流出后幾分鐘內流出,若肽鏈很小,洗脫太早,多肽的量將很低。
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