VECTOR免疫組化試劑盒VECTASTAIN ABC Kit(PK-6100)
Vector Laboratories公司是世界著名的生物學檢測試劑公司。1977年首先開發出了生物素-親和素系統,該產品系列是檢測方法上的重要革命。之后經過不懈的努力,VectorLaboratories公司發展出ABC技術(Avidin : Biotinylated Enzyme ComplexTechnology,親和素-生物素-過氧化物酶復合物技術)并建立了著名的VECTASTAIN@ ABC系統,該系統被普遍視為目前zui靈敏、zui可靠與zui有效的染色系統,并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。而該系統仍在不斷地發展,以滿足廣大研究人員的各種不同要求(如多抗原的標記檢測)
VECTOR免疫組化試劑盒VECTASTAIN ABC Kit(PK-6100)
ABC技術利用生物素和卵白素具有*親和性的生物學特征,將卵白素和生物素化辣根酶按照一定的比例混合,形成ABC復合物。該法亦被稱作三步法,*步為biotin化二抗和一抗結合,第二步為avidin(親和素,A液)和二抗上的biotin結合,第三步為HRP偶聯的biotin(B液)和avidin結合,而HRP再催化底物顯色完成染色。
VECTOR免疫組化試劑盒VECTASTAIN ABC Kit(PK-6100)
Features:
- Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
- HIghest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximay 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
- Cost effective: HIgher sensitivity leads to lower cost per slide
- Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
- Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.